巴西细菌和噬菌体生物学研究中心招聘博士后—结构生物学

巴西细菌和噬菌体生物学研究中心招聘博士后—结构生物学

Post-Doctoral Fellowship in Structural Biology

Biology of Bacteria and Bacteriophages Research Centre

Date Posted: Posted on 19 August 2025

Location: Brazil

Salary: $27,430 - $27,430

Job Tags:

Biophysics, Nuclear magnetic resonance

A postdoctoral position is available to work in the Laboratory of Biomolecular

NMR Spectroscopy within the Research, Innovation, and Dissemination Centre B3 (Biology

of Bacteria and Bacteriophages), supported by FAPESP. Bacterial Type IV Secretion

Systems (T4SSs) are nanomachines that transport effector proteins or protein-DNA

complexes from the cytoplasm to the extracellular milieu or to a host cell. The Xanthomonas

citri T4SS (T4SSXAC) belongs to the class of minimized systems, which consist of eleven

VirB subunits (VirB1 to VirB11) and the coupling protein VirD4. All T4SSs exhibit an outer

membrane associated core complex (OMCC), which is connected by a stalk or a channel to

the inner membrane complex (IMC). In minimized systems, the IMC is composed of the

VirB10 transmembrane helix, and the membrane proteins VirB3, VirB4, VirB6, and VirB8.

While atomic-level three-dimensional structures of the OMCC from expanded and minimized

T4SSs were obtained using electron cryomicroscopy (Cryo-EM), the IMC has been resistant

to isolation and structural characterization. The aim of this project is to develop protocols to

express and purify transmembrane domains of Type IV Secretion System (T4SS) subunits

(VirB6 and VirB8), followed by their structural or biophysical characterization in isolation

using biochemistry tools, NMR spectroscopy and electron microscopy (Cryo-EM or negative

staining) techniques. The successful candidate will be a motivated individual holding a PhD

in biochemistry, structural biology or related areas and experience in protein expression and

purification of membrane proteins. Prior experience in NMR or electron microscopy is not

necessary. Applications should be submitted by email until

September 5 th , 2025, accompanied by the following documents:

– A letter of motivation, describing the reasons for the interest in this position and

previous academic experience;

– One recommendation letter;

– A short CV indicating a link to Google Scholar, Research ID or ORCID profile.

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