美国凯斯西储大学医学院蛋白质分析及相关领域博士后职位

美国凯斯西储大学医学院蛋白质分析及相关领域博士后职位

We are looking to add 2 postdoctoral co-worker(s) to our interdisciplinary team as new NIH funding has come to our laboratory for the investigation of the role of Plexin in interacting with kinases and Alzheimer proteins . Our interest is to understand these protein-protein interactions at the protein biophysics, biochemical, structural and protein dynamics level {e.g. see recent papers of our laboratory projects and approaches ACS Central Science 2018 doi: 10.1021/acscentsci.7b00593 ; J.Biol.Chem. 2019 doi: 10.1074/jbc.RA118.004021; Cell. Mol. Life Sci. 2020, doi: 10.1007/s00018-020-03571-2 and bioRxiv 2020 doi: 10.1007/s00018-020-03571-2}.

We principally use solution NMR, not so much for protein structure determination but more for protein surface and interaction site mapping and characterization of protein dynamics and conformational change. The project may also involve amide hydrogen exchange on a state of the art Waters mass spectrometer dedicated to HX-MS, and employ NMR methyl relaxation and molecular dynamics simulation as additional tools. The NorthEast Ohio High Field NMR facility consists of a 600 MHz, 700 MHz, 800 MHz and a 900 MHz Bruker spectrometers. With the recent installation of a Titan Krios at Case Western there is also considerable interest to apply cryo-EM to our systems and a postdoc will be able to learn from our local collaborator and facility manager.

We are looking for talented and highly motivated coworkers, who aim to become an independent researcher/project leader (> ½ of the previous postdocs accomplished this goal). Prior experience with protein NMR is essential. The Buck laboratory provides a stimulating but friendly working environment. The Medical School of Case Western Reserve University is highly rated overall in the US (~ 16th. in terms of NIH funding for 2020) and Cleveland is a pleasant but affordable place to live with excellent educational, medical and cultural institutions. The starting date is as soon as possible but this project details and salary are negotiable. Please send your resume, overview of your scientific interests and career goals as well as contact details of three referees able to provide references by e-mail to matthias.buck@case.edu