ERC-Funded Postdoctoral Researcher In Single-Cell Immunology
Karolinska Institute Stockholm
We are looking for an ambitious postdoc with solid single-cell immunology skills to join our highly accomplished team of immunologists in a project ( GUT-SEQ ) funded by the European Research Council (ERC). We offer a stimulating environment in a rapidly moving research frontier.
The Mjösberg group (https: // mjosberglab.wixsite.com/ilcs and https: // ki.se/en/medh/jenny-mjosberg-group) studies the development, regulation and function of innate lymphoid cells (ILCs), in humans, and how these cells contribute to pathology in mucosal organs such as the lung and gut. The studies are translational as they involve advanced basic molecular and cellular immunological investigations of precious human tissue samples to discover novel disease mechanisms and therapy targets. The group is internationally recognized as a leader in the field through several high impact publications in recent years (Nat Immunol 2016, Nat Comm 2020, Cell Res 2020 etc).
The postdoc will continue the successful line of investigations utilizing single-cell RNA sequencing (scRNA-seq) to dissect human intestinal mucosal ILC and T cell biology in health and disease. The successful candidate will be part of a team in an ongoing project that involves dissection of gut-associate lymphoid tissues from human intestinal specimens and generation of scRNA-seq(SMARTseq and 10X genomics) data as well as multicolor flow cytometry data of human mucosal ILCs and T cells from these tissues. The data will be analyzed in close collaboration with bioinformaticians at SciLifeLab. As this is a team effort, excellent communication skills are essential, as is proven ability to work in multidisciplinary teams. It is expected that the postdoc also supervises students and other researchers if necessary, as well as contribute to common lab duties.
Qualified to be employed as a postdoctor is one who has obtained a doctorate or has equivalent scientific competence. Applicants who have not completed a doctorate at the end of the application period may also apply, provided that all requirements for a completed degree are met before the (intended) date of employment. This must be substantiated by the applicant's main supervisor, director or equivalent.
The candidate must hold a PhD equivalent to a Swedish doctoral degree in the field of immunology. The candidate must fully master multidimensional (>20 parameters) flow cytometry analysis and sorting, as well as be experienced in scRNAseq. Experience in isolation and in vitro culturing of primary immune cells is a requirement, as is basic functional in vitro assessments of immune cell function such as intracellular cytokine stainings, ELISA etc. The candidate should be familiar with R software and have some experience analyzing scRNAseq data. The candidate should have a strong publication track- record. The candidate should be self-motivated, have good communications skills and a proven ability to interact effectively and work productively in an interdiciplinary team. Fluency in English is a requirement as well as some basic skills and willingness to learn Swedish.