英国布里斯托大学蛋白质设计与合成生物学博士后职位

英国布里斯托大学蛋白质设计与合成生物学博士后职位

Research Associate in protein design and synthetic biology

University of Bristol

Job Description

Research AssociateJob number

ACAD104713

Division/School

School of Chemistry

Contract type

Open Ended

Working pattern

Full time

Salary

£33,797 - £38,017 per annum

Closing date for applications

14-Oct-2020

The group of Dr Fabio Parmeggiani (School of Chemistry and School of Biochemistry, University of Bristol) has an opportunity for a talented and enthusiastic postdoc interested in protein design and synthetic biology.

This post is available for 2 years in the first instance.

The project will be focused on designing custom modular protein architectures as robust and reliable scaffolds for the development of novel functional protein-based nanomaterials and multivalent ligands to modulate cell response.

The project will build on work on computational design of repeat proteins (Parmeggiani et al.J. Mol. Biol. , 2015, 427 , 563-575, DOI: 10.1016/j.jmb.2014.11.005 ; Brunette et al.Nature , 2015, 528 , 580-584 DOI: 10.1038/nature16162 ; Fallas et al.Nat. Chem. , 2017, 9 , 353-360 DOI: 10.1038/nchem.2673) and their use as building blocks for design of novel proteins with custom shapes (Park et al.Nat. Struct. Mol. Biol. , 2015, 22 , 167-174 DOI: 10.1038/nsmb.2938 ; Yeh et al.J. Struct. Biol. , 2018, 201 , 100-107. DOI: 10.1016/j.jsb.2017.09.001).

The aim of the project is to develop a robust, fast and high throughput design platform to rapidly build new proteins and complexes. The researcher will be responsible for developing the experimental part of the pipeline (involving DNA assembly, protein expression and purification, validation of designs through biophysical and structural characterization) and spearhead applications in nanomaterials and cell biology.

The position would be best suited to a researcher with a keen interest in applying de novoprotein design in synthetic biology. Essential skills for this role would include: design, construction and expression of synthetic genes in E. coli ; biochemical and biophysical characterisation of proteins; strong structural biology expertise, in particular with cryo-electron microscopy and x-ray crystallography.

Experiences in protein modelling, computational biology and programming are desirable, but not required.

For informal queries, please contact Fabio Parmeggiani – fabio.parmeggiani@bristol.ac.uk